Journal: Annals of Biomedical Engineering

Article Title: Perfusion Flow Enhances Viability and Migratory Phenotype in 3D-Cultured Breast Cancer Cells

doi: 10.1007/s10439-021-02727-w

Figure Lengend Snippet: Comparison of cell viability and proliferation of MDA-MB-231 cells grown in 3D collagen scaffolds in static and dynamic cell culture conditions. (a) Cell counts at 2, 4 and 7 days of culture in static and dynamic conditions, (b) and dead cell percentages. Mean values and ± SEM of cell counts of at least 4–6 scaffolds per condition, cultured in at least 2 independent experiments. * p < 0.05.

Article Snippet: Briefly, RatCol ® Rat Tail Collagen for 3D Hydrogels (Advanced BioMatrix, Carlsbad, CA, USA) and its neutralization solution were mixed in the proportion 9:1 keeping the solution in ice to prevent polymerization.

Techniques: Comparison, Cell Culture

Journal: Annals of Biomedical Engineering

Article Title: Perfusion Flow Enhances Viability and Migratory Phenotype in 3D-Cultured Breast Cancer Cells

doi: 10.1007/s10439-021-02727-w

Figure Lengend Snippet: Scratch wound healing assay in MDA-MB-231 cells grown in 3D collagen scaffold for 7 days in static and dynamic conditions and seeded in standard tissue culture plates after collagen digestion. Images were captured at 0, 2, 4, 6, 8 and 24 h post-wound (at least 8 images per condition with cells obtained from at least 3 different scaffolds). Cell-free surface area was calculated over time and fitted with an exponential curve (grey squares/curve and black circles/curve for static and dynamic conditions, respectively). * p < 0.05; ** p < 0.01.

Article Snippet: Briefly, RatCol ® Rat Tail Collagen for 3D Hydrogels (Advanced BioMatrix, Carlsbad, CA, USA) and its neutralization solution were mixed in the proportion 9:1 keeping the solution in ice to prevent polymerization.

Techniques: Wound Healing Assay

Journal: Annals of Biomedical Engineering

Article Title: Perfusion Flow Enhances Viability and Migratory Phenotype in 3D-Cultured Breast Cancer Cells

doi: 10.1007/s10439-021-02727-w

Figure Lengend Snippet: Gene expression analysis of MDA-MB-231 cells grown in 3D collagen scaffold (mRNA collected from 3 scaffolds per condition cultured in 2 independent experiments) for 4 days in static and dynamic conditions. The mRNA of invasion/migration related genes LOX , MMP2-3-9 , RHOA , and VIM were analyzed by qPCR using ACTB and HPRT1 as reference genes (2 −ΔΔCT method). Fold changes respect to static condition were calculated.* p < 0.05; ** p < 0.01.

Article Snippet: Briefly, RatCol ® Rat Tail Collagen for 3D Hydrogels (Advanced BioMatrix, Carlsbad, CA, USA) and its neutralization solution were mixed in the proportion 9:1 keeping the solution in ice to prevent polymerization.

Techniques: Gene Expression, Cell Culture, Migration